Chiyoda’s successful development of freeze-thaw treatment for the separation and purification of genetically modified proteins in tobacco leaves
Information Jan 16, 2024
A research article has been published in the international journal of experimental plant biology, ‘Plant Science’
A research article ‘Removal of plant endogenous proteins from tobacco leaf extract by freeze–thaw treatment for purification of recombinant proteins’, co-authored by Chiyoda and the National Institute of Advanced Industrial Science and Technology (AIST) was published in ‘Plant Science’ in December 2023.
Following the New Energy and Industrial Technology Development Organization (NEDO) open call for proposals on Biomanufacturing for the production of materials in plants using gene recombination technology in 2022, Chiyoda was selected to develop large-scale production technology to expand the practical use of substances that require a high degree of modification, uncommercialized in recombinant plants to date.
220822_e.pdf (chiyodacorp.com)
We subsequently developed freeze-thaw treatment to separate and purify recombinant and contaminant proteins, such as rubisco, in tobacco leaves.
A research article was co-authored by Mr. Akito Matsushima (Frontier Business Division, Chiyoda Corporation) and Mr. Kouki Matsuo (National Institute of Advanced Industrial Science and Technology (AIST), Bioproduction Research Institute) based on their mutual cooperation.
https://www.sciencedirect.com/science/article/pii/S0168945223003709
The technology is universally applicable for the production of substances using tobacco leaves and we anticipate further simplification of the refining process to reduce refining durations and costs.
Utilizing our technology as the basis to commercialize plant material production technology and contribute to the development of plant-based material production industries, Chiyoda will commence constructing plant bio foundries in 2025.
Further information regarding the research article:
Publisher: Plant Science
Title: Removal of plant endogenous proteins from tobacco leaf extract by freeze–thaw treatment for purification of recombinant proteins
Authors: Akito Matsushima, Kouki Matsuo